Assuntos
Antígenos de Grupos Sanguíneos/genética , Transfusão de Sangue , Técnicas de Genotipagem , Reação em Cadeia da Polimerase em Tempo Real/métodos , Adulto , Alelos , Tipagem e Reações Cruzadas Sanguíneas/métodos , Brasil , DNA/sangue , DNA/genética , Primers do DNA , Feminino , Frequência do Gene , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Polimorfismo de Fragmento de Restrição , Padrões de Referência , Sensibilidade e Especificidade , Adulto JovemAssuntos
Doadores de Sangue , Hemofilia A/virologia , Parvovirus/isolamento & purificação , Parvovirus/fisiologia , Voluntários , Talassemia beta/virologia , Adolescente , Adulto , Brasil , Criança , Pré-Escolar , Feminino , Hemofilia A/sangue , Humanos , Lactente , Masculino , Pessoa de Meia-Idade , Reação Transfusional , Adulto Jovem , Talassemia beta/sangueAssuntos
Bancos de Sangue , Transfusão de Sangue , DNA Viral/sangue , Eritema Infeccioso , Hemofilia A/terapia , Parvovirus B19 Humano , Adolescente , Adulto , Doadores de Sangue , Brasil/epidemiologia , Criança , Seleção do Doador , Eritema Infeccioso/sangue , Eritema Infeccioso/transmissão , Feminino , Hemofilia A/epidemiologia , Humanos , Masculino , Pessoa de Meia-Idade , Estudos SoroepidemiológicosRESUMO
Interleukin-18 (IL-18) and interferon-gamma (IFN-γ) exert important functions in both innate and adaptive immune responses against intracellular pathogens and viruses. Previous studies suggested that host genetic factors, including cytokines gene polymorphisms, could be involved in the pathogenesis of human T-cell leukemia virus type 1 (HTLV-1)-associated myelopathy/tropical spastic paraparesis (HAM/TSP). Thus, we analyzed -137C/G and -607A/C of the IL-18 promoter and +874T/A of the IFN-γ in DNA samples from 98 HTLV-1-infected individuals exhibiting or not clinical symptoms and 150 healthy control individuals. The IL-18 promoter -607CC genotype was significantly lower in HTLV-1 asymptomatic carriers (HAC) and HTLV-1-infected individuals (HAC + HAM/TSP) than healthy control group. In contrast, the -607AC genotype was significantly higher in HAC and HTLV-1-infected individuals group compared to the healthy control group. The -137G/-607A IL-18 haplotype was higher in infected group than healthy control group, and the -137C/-607C IL-18 haplotype was increased in the healthy control group compared to the others. Finally, the IFN-γ polymorphism analysis showed that the HTLV-1-infected individuals with +874AT genotype presented higher proviral load than +874AA genotype. These data indicate that the IL-18-607AC genotype and -137G/-607A haplotype could be a risk factor for HTLV-1 infection, whereas the protective effect could be conferred by -607CC genotype and -137C/-607C haplotype. Also, the IFN-γ could be implicated on the proviral load levels.
